Analysis of platelet eluate for the elucidation of sensitization to HLA in kidney transplant candidate

Hugo Mendonça Mundim, Maria Aparecida Caixeta Lins, Eridane Botelho Guzmán, Heline Leal Titan, Patrícia Keiko Saito, Sueli Donizete Borelli


While a 42-year-old male patient was being prepared for deceased-donor renal transplantation, anti-HLA-A2 antibodies were detected in the serum by enzyme-linked immunosorbent assay (ELISA) method. The patient denied any transfusion history and previous transplant. Crossmatch by complement dependent cytotoxicity (CDC) and CDC with anti-human globulin (CDC-AHG) proved negative with a four-cell panel with positive typing for HLA-A2. Adsorption of antibodies with platelets and analysis of eluate were suggested to elucidate discrepancies in results by ELISA and by CDC-AHG. ELISA showed that adsorbed serum with platelets did not reveal antibodies for HLA-A2 specificity and suggested that they were removed by their specific binding with HLA-A2 antigens on the platelet surface. Eluate analysis by ELISA showed antibodies for HLA-A2 specificity. No antibodies for HLA-A2 specificity in the non-adsorbed serum were detected by CDC-AHG method. Revision of patient’s data showed that a previous transfusion had occurred, which may have been the source of HLA sensitization. The suggested method may be a contribution towards the evaluation of sensitivity between CDC-AHG and ELISA methods for characterizing antibodies in the patient’s serum.


antibodies; immunologic cytotoxicity tests; enzyme-linked immunosorbent assay; HLA Antigens; kidney transplantation

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ISSN 1679-9291 (impresso) e ISSN 1807-8648 (on-line) e-mail:


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